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Effect of fulvic acid induction on the physiology, metabolism, and lipid biosynthesis-related gene transcription of Monoraphidium sp. FXY-10

Che, Raoqiong, Huang, Li, Xu, Jun-Wei, Zhao, Peng, Li, Tao, Ma, Huixian, Yu, Xuya
Bioresource technology 2017 v.227 pp. 324-334
Monoraphidium, acetyl-CoA carboxylase, biofuels, carbohydrate content, enzyme activity, fulvic acids, gene expression, gene expression regulation, genes, lipid content, lipids, malic enzyme, metabolism, microalgae, phosphoenolpyruvate carboxylase, protein content, reactive oxygen species, transcription (genetics)
Fulvic acid (FA) triggers lipid accumulation in Monoraphidium sp. FXY-10, which can produce biofuels. Therefore, the metabolism shift and gene expression changes influenced by fulvic acid should be investigated. In this study, lipid and protein contents increased rapidly from 44.6% to 54.3% and from 31.4% to 39.7% under FA treatment, respectively. By contrast, carbohydrate content sharply declined from 49.5% to 32.5%. The correlation between lipid content and gene expression was also analyzed. Results revealed that accD, ME, and GPAT genes were significantly correlated with lipid accumulation. These genes could likely influence lipid accumulation and could be selected as modification candidates. These results demonstrated that FA significantly increased microalgal lipid accumulation by changing the intracellular reactive oxygen species, gene expression, and enzyme activities of acetyl-CoA carboxylase, malic enzyme, and phosphoenolpyruvate carboxylase.