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The direct action of hyaluronic acid on human U-937 and HL-60 cells – modification of native and model membranes

Barbasz, Anna, Kreczmer, Barbara, Dyba, Barbara, Filek, Maria, Rudolphi-Skórska, Elżbieta
Biologia 2016 v.71 no.11 pp. 1304-1314
cell viability, cytotoxicity, granulocytes, humans, hyaluronic acid, lipids, macrophages, molecular weight, receptors
The aim of the study was to investigate the effect of application of exogenous hyaluronic acid (HA) of various molecular weights on the cells of human immune system. Two cell lines HL-60 and U-937 with various ability to differentiation were chosen. HL-60 cells were differentiated to macrophages and granulocytes, whereas U-937 only to macrophages. For all investigated cell systems the most cytotoxic effect, indicated as a decrease of cell viability, was found at HA dose equal to 200 mg/L. However, greater effect was observed for differentiated cells and at longer exposure to HA. The possibility of HA interaction with both specific receptors and membrane lipids was tested by determination of biotin-labelled HA binding to cell surface and analysis of physicochemical parameters of model membranes.