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Effects of different pretreatment methods on the enzymatic hydrolysis of oak shell

Yang, Jing, Jiang, Jianchun, Zhang, Ning, Wei, Min, Zhao, Jian
International journal of green energy 2017 v.14 no.1 pp. 33-38
Fourier transform infrared spectroscopy, Quercus acutissima, X-ray diffraction, cellulose, delignification, enzymatic hydrolysis, enzymatic treatment, ethanol production, glucose, hemicellulose, saccharification, scanning electron microscopy, sodium hydroxide, sulfuric acid
Sawtooth Oak (Quercus acutissima) shells were used as a renewable and low-cost agricultural residue for bioethanol production for the first time. The efficiency of H ₂SO ₄, NaOH, steam explosion and the combination of these methods was compared in terms of delignification, saccharification efficiency and yield. The structural features of samples were characterized by SEM, XRD and FTIR. Results show H ₂SO ₄/steam explosion resulted in the highest hemicellulose reduction (98.5%) and cellulose recovery yield (99.9%). NaOH /steam explosion resulted in the highest delignification level (31.5%). Steam explosion exhibited the highest enzymatic digestibility of 98.8% and total product yield of glucose of 84.8%, an increase of 130.8% and 98.1% than that of untreated oak shell, respectively, which seemed to be the most effective for improving enzymatic saccharification. The results of structural features showed the structure and surface of shells were changed that is in favor of the following enzymatic hydrolysis.