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Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Isanga, Joel, Tochi, Bitange Nipa, Mukunzi, Daniel, Chen, Yanni, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai
Food and agricultural immunology 2017 v.28 no.1 pp. 49-66
aminoglycosides, apramycin, cross reaction, detection limit, enzyme-linked immunosorbent assay, foods, inhibitory concentration 50, monoclonal antibodies
A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC ₅₀) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02–105.49%, with coefficients of variation in the range of 2.21–11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5–5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.