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Development of a specific monoclonal antibody assay and a rapid testing strip for the detection of apramycin residues in food samples

Author:
Isanga, Joel, Tochi, Bitange Nipa, Mukunzi, Daniel, Chen, Yanni, Liu, Liqiang, Kuang, Hua, Xu, Chuanlai
Source:
Food and agricultural immunology 2017 v.28 no.1 pp. 49-66
ISSN:
1465-3443
Subject:
aminoglycosides, apramycin, cross reaction, detection limit, enzyme-linked immunosorbent assay, foods, inhibitory concentration 50, monoclonal antibodies
Abstract:
A specific monoclonal antibody (MAb) against apramycin (AP) was produced and used to develop an indirect competitive enzyme-linked immunosorbent assay (idcELISA) and a rapid testing strip for the detection of AP residues in foods. MAb exhibited negligible cross-reactivity with other aminoglycosides. Under optimized conditions in 0.01 M PBS, the half maximum inhibitory concentration (IC ₅₀) of MAb was 0.41 ng/ml with a limit of detection (LOD) of 0.15 ng/ml. The ELISA results were obtained within 90 min. The mean recoveries from all the spiked food samples were within the range of 79.02–105.49%, with coefficients of variation in the range of 2.21–11.4%. The strip test results obtained within 5 min had visual LODs in the range 2.5–5 µg/kg (ng/ml) for all food samples tested. Therefore, the developed strip test represents a fast and convenient detection method of AP residues in foods.
Agid:
5608437