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Predicting the Presence of Non-O157 Shiga Toxin-Producing Escherichia coli in Ground Beef by Using Molecular Tests for Shiga Toxins, Intimin, and O Serogroups
- Bosilevac, Joseph M., Koohmaraie, Mohammad
- Applied and environmental microbiology 2012 v.78 no.19 pp. 7152
- Shiga toxin, Shiga toxin-producing Escherichia coli, bacterial contamination, food contamination, food microbiology, genes, ground beef, intimin
- The USDA Food Safety and Inspection Service has recently announced its intention to consider six non-O157 Shiga toxin-producing Escherichia coli (STEC) O26, O45, O103, O111, O121 and O145 as adulterants in certain beef products. Most of the current methods for the detection of these organisms are based on molecular detection of Shiga toxin, intimin and O group specific genes in an enriched beef sample. We report here the results of applying this sort of detection scheme to a previously characterized set of 3,972 samples and determined the numbers of samples that would be identified and properly predict the presence of adulterant STEC. Whereas only six non-O157 STEC containing samples were known to be present in the set, this approach identified 183 samples as presumptive positive while failing to identify 4 of the adulterant containing samples.