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Prevalence of pathogens from Mollicutes class in cattle affected by respiratory diseases and molecular characteristics of Mycoplasma bovis field strains

Szacawa, Ewelina, Szymańska-Czerwińska, Monika, Niemczuk, Krzysztof, Dudek, Katarzyna, Woźniakowski, Grzegorz, Bednarek, Dariusz
Journal of Veterinary Research 2016 v.60 no.4 pp. 391-397
DNA, Mycoplasma bovirhinis, Mycoplasma bovis, Ureaplasma diversum, bacteria, cattle, denaturing gradient gel electrophoresis, genes, herds, mutation, nose, pathogens, phylogeny, pneumonia, random amplified polymorphic DNA technique, sequence analysis
Introduction: Mycoplasma bovis is one of the main pathogens involved in cattle pneumonia. Other mycoplasmas have also been directly implicated in respiratory diseases in cattle. The prevalence of different Mycoplasma spp. in cattle affected by respiratory diseases and molecular characteristics of M. bovis field strains were evaluated. Material and Methods: In total, 713 nasal swabs from 73 cattle herds were tested. The uvrC gene fragment was amplified by PCR and PCR products were sequenced. PCR/DGGE and RAPD were performed. Results: It was found that 39 (5.5%) samples were positive for M. bovis in the PCR and six field strains had point nucleotide mutations. Additionally, the phylogenetic analysis of 20 M. bovis field strains tested with RAPD showed two distinct groups of M. bovis strains sharing only 3.8% similarity. PCR/DGGE analysis demonstrated the presence of bacteria belonging to the Mollicutes class in 79.1% of DNA isolates. The isolates were identified as: Mycoplasma bovirhinis, M. dispar, M. bovis, M. canis, M. arginini, M. canadense, M. bovoculi, M. alkalescens, and Ureaplasma diversum. Conclusion: Different Mycoplasma spp. strains play a crucial role in inducing respiratory diseases in cattle.