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Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

Author:
Budniak, Sylwia, Kędrak-Jabłońska, Agnieszka, Szczawińska, Anna, Reksa, Monika, Krupa, Marek, Szulowski, Krzysztof
Source:
Journal of Veterinary Research 2016 v.60 no.4 pp. 411-416
ISSN:
2450-8608
Subject:
Listeria monocytogenes, bacteria, blood, brain, liver, polymerase chain reaction
Abstract:
Introduction: The aim of the study was to optimise and compare two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples including the liver, brain, and blood. Material and Methods: Three strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used. Additionally, five other species of bacterium were used to evaluate the specificity of the tests. Results: Specific amplification products were obtained for both multiplex PCR assays, which confirmed the tested strains as Listeria spp. and L. monocytogenes, respectively. Isolates of other species did not yield PCR products. Conclusion: Both multiplex PCR assays proved to be significantly sensitive and highly-specific methods for the detection of Listeria strains.
Agid:
5608902