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Characterisation of Staphylococcus aureus isolated from meat processing plants – a preliminary study

Kizerwetter-Świda, Magdalena, Chrobak-Chmiel, Dorota, Rzewuska, Magdalena, Pławińska-Czarnak, Joanna, Binek, Marian
Journal of Veterinary Research 2016 v.60 no.4 pp. 441-446
antibiotic resistance, beef, enterotoxins, genes, genetic relationships, livestock, meat processing plants, methicillin, methicillin-resistant Staphylococcus aureus, multiple drug resistance, pulsed-field gel electrophoresis, raw meat, risk, Poland
Introduction: Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) belonging to the clonal complex 398 (CC398) emerged recently in livestock as a new type of MRSA, which may cause zoonotic infections. This study presents data on the characterisation of S. aureus isolated from the meat processing plants. Material and Methods: S. aureus was isolated from 90 samples collected in the raw meat warehouse, from devices and surfaces of meat processing plants, and from finished meat products. The isolates were subjected to molecular analysis in order to investigate the presence of enterotoxin genes, the mecA gene, and to verify whether they belong to the clonal complex 398. The genetic relatedness of the isolates was determined using pulsed-field electrophoresis. Likewise, antimicrobial susceptibility was tested. Results: From 21 S. aureus strains isolated, five belonged to the CC398, two of which were recognised as MRSA and three as methicillin-sensitive Staphylococcus aureus (MSSA). The most prevalent enterotoxin genes were seg and sei. Two MRSA CC398 isolates, three MSSA CC398, and one MSSA were classified as multidrug-resistant. Conclusion: The first isolation of MSSA CC398 from beef in Poland indicates contamination of beef by strains belonging to this clonal complex. The occurrence of multidrug-resistant enterotoxigenic S. aureus isolates in the finished meat products constitutes a potential risk for the consumers.