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The N-butyl alcohol extract from Hibiscus rosa-sinensis L. flowers enhances healing potential on rat excisional wounds

Author:
Shen, Hui-Min, Chen, Chun, Jiang, Ji-Yang, Zheng, Yi-Lin, Cai, Wen-Feng, Wang, Bin, Ling, Zhen, Tang, Liu, Wang, Yuan-Hang, Shi, Gang-Gang
Source:
Journal of ethnopharmacology 2017 v.198 pp. 291-301
ISSN:
0378-8741
Subject:
Hibiscus rosa-sinensis, alcohols, angiogenesis, antioxidants, carbon, cattle, cell death, collagen, death, dimethyl sulfoxide, fibroblast growth factor 2, fibroblasts, flowers, histopathology, immunohistochemistry, immunomodulation, leaves, low density lipoprotein, macrophages, methylcellulose, models, oxidation, rats, staining, tissue repair, traditional medicine, transforming growth factor beta 1, vascular endothelial growth factors, China
Abstract:
Hibiscus rosa-sinensis L. (HRS), a folk medicine named Zhujin in China, possess anti-tumor, antioxidant, antibacterial, low density lipoprotein oxidation prevention and macrophage death prevention effects. The leaves and red flowers of HRS have been traditionally used to treat with furuncle and ulceration.To investigate the efficacy and possible mechanism of the N-butyl alcohol extract of HRS (NHRS) red flowers in wound healing by analyzing the collagen fiber deposition, angiogenic activity and macrophages action of the NHRS.In an excisional wound healing model in rats, different concentrations of NHRS, or recombinant bovine basic fibroblast growth factor (rbFGF), were respectively applied twice daily for 9 days. Histopathology was assessed on day 9 via hematoxylin and eosin (HE) and Masson's trichrome (MT) staining, and immunohistochemistry for vascular endothelial growth factor (VEGF), transforming growth factor-β1 (TGF-β1) and CD68. Immunomodulation by NHRS was evaluated by a carbon clearance test in mice.Wound healing post-surgery was greater in the rbFGF-control, NHRS-M and MHRS-H groups than in the model and 5% dimethylsulfoxide (DMSO)-control groups after the third day. By the sixth day the wound contraction of NHRS-M and MHRS-H groups was much higher than the rbFGF-control group. HE and MT staining revealed that epithelialization, fibroblast distribution, collagen deposition of NHRS-M- and NHRS-H-control groups were significantly higher than the model group. Moreover, immunohistochemistry showed more intense staining of VEGF, TGF-β1 and CD68 in the rbFGF- and NHRS-control groups, compared to that in model and 5% DMSO-control groups. The clearance and phagocytic indices of NHRS-M- and NHRS-H-control groups were significantly higher than that of the carboxyl methyl cellulose (CMC) group in mice.NHRS accelerates wound repair via enhancing the macrophages activity, accelerating angiogenesis and collagen fiber deposition response mediated by VEGF and TGF-β1.
Agid:
5613109