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Gigantol from Dendrobium chrysotoxum Lindl. binds and inhibits aldose reductase gene to exert its anti-cataract activity: An in vitro mechanistic study

Author:
Wu, Jie, Li, Xue, Wan, Wencheng, Yang, Qiaohong, Ma, Weifeng, Chen, Dan, Hu, Jiangmiao, Chen, C.-Y.Oliver, Wei, Xiaoyong
Source:
Journal of ethnopharmacology 2017 v.198 pp. 255-261
ISSN:
0378-8741
Subject:
Dendrobium chrysotoxum, Oriental traditional medicine, Raman spectroscopy, aldehyde reductase, antioxidants, cataract, epithelial cells, gene expression, genes, glucose, glycemic effect, reverse transcriptase polymerase chain reaction, ultraviolet-visible spectroscopy
Abstract:
Dendrobium. chrysotoxum Lindl is a commonly used species of medicinal Dendrobium which belongs to the family of Orchidaceae, locally known as “Shihu” or “Huangcao”. D. chrysotoxum Lindl is widely known for medicinal values in traditional Chinese medicine as it possesses anti-inflammatory, anti-hyperglycemic induction, antitumor and antioxidant properties.To characterize the interaction between gigantol extracted from D. chrysotoxum Lindl and the AR gene, and determine gigantol's efficacy against cataractogenesis.Human lens epithelial cells (HLECs) were induced by glucose as the model group. Reverse transcription polymerase chain reaction (RT-PCR) was used to assess AR gene expression. Then, the mode of interaction of gigantol with the AR gene was evaluated by UV–visible spectroscopy, atomic force microscope (AFM) and surface-enhanced Raman spectroscopy (SERS). The binding constant was determined by UV–visible.Gigantol depressed AR gene expression in HLECs. UV–visible spectra preliminarily indicated that interaction between the AR gene and gigantol may follow the groove mode, with a binding constant of 1.85×10³L/mol. Atomic force microscope (AFM) data indicated that gigantol possibly bound to insert AR gene base pairs of the double helix. Surface-enhanced Raman spectroscopy (SERS) studies further supported these observations.Gigantol extracted from D. chrysotoxum Lindl not only has inhibitory effects on aldose reductase, but also inhibits AR gene expression. These findings provide a more comprehensive theoretical basis for the use of Dendrobium for the treatment of diabetic cataract.
Agid:
5613111