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Detection of antibodies against hepatitis B virus surface antigen and hepatitis C virus core antigen in plasma with a waveguide-mode sensor

Author:
Shimizu, Takenori, Tanaka, Torahiko, Uno, Shigeyuki, Ashiba, Hiroki, Fujimaki, Makoto, Tanaka, Mutsuo, Awazu, Koichi, Makishima, Makoto
Source:
Journal of bioscience and bioengineering 2017 v.123 no.6 pp. 760-764
ISSN:
1389-1723
Subject:
Hepatitis B virus, Hepatitis C virus, antibodies, antigen-antibody reactions, blood, blood groups, blood sampling, blood transfusion, colorimetry, detection limit, disasters, earthquakes, electric power, hepatitis C antigens, horseradish, humans, infectious diseases, mice, peroxidase, reflectance spectroscopy, screening, surface antigens, surgery, traffic, viruses
Abstract:
In large-scale disasters, such as huge significant earthquakes, on-site examination for blood typing and infectious disease screening will be very helpful to save lives of victims who need surgical treatment and/or blood transfusion. However, physical damage, such as building collapse, electric power failure and traffic blockage, disrupts the capacity of the medical system. Portable diagnostic devices are useful in such cases of emergency. In this study, we evaluated a waveguide-mode sensor for detection of anti-hepatitis virus antibodies. First, we examined whether we can detect antigen–antibody interaction on a sensor chip immobilized hepatitis B virus surface (HBs) antigen and hepatitis C virus (HCV) core antigen using monoclonal mouse antibodies for HBs antigen and HCV core antigen. We obtained significant changes in the reflectance spectra, which indicate specific antigen–antibody interaction for anti-HBs antibody and anti-HCV antibody. Next, we examined the effect of horseradish peroxidase-conjugated secondary antibody using aminoethyl carbazole as the peroxidase substrate and found that the colorimetric reaction increases detection sensitivity for anti-HBs antibody more than 300 times. Finally, we successfully detected anti-HBs antibody in human blood samples with an enhancing method using a peroxidase reaction. Thus, a portable device utilizing a waveguide-mode sensor may be applied to on-site blood testing in emergency settings.
Agid:
5614551