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The role of pi-interactions and hydrogen bonds in fully protective synthetic malaria vaccine development

Author:
Reyes, César, Moreno-Vranich, Armando, Patarroyo, Manuel Elkin
Source:
Biochemical and biophysical research communications 2017
ISSN:
0006-291X
Subject:
Plasmodium falciparum, databases, hydrogen bonding, immune response, malaria, malaria vaccines, parasites, peptides, vaccine development
Abstract:
Analysis of our Plasmodium falciparum malaria parasite peptides' 1H-NMR database in the search for H-bonds and π-interactions led us to correlate their presence or absence with a peptide's particular immunological behavior. It was concluded that a 26.5 ± 1.5 Å between positions 1 to 9 of the HLA-DRβ1* interacting region was necessary for proper docking of 20mer-long peptides and these MHC Class II molecules for full-protective immunity. Presence of intramolecular H-bonds or π-interactions leading to righ-handed α-helix or β-turn conformation in this peptide's region induces different immune responses or none. PPIIL conformation and the absence of any intramolecular interaction thus became the first feature characterising our immune protection-inducing structures as malaria vaccine candidates.
Agid:
5617208