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Protein adsorption of dialdehyde cellulose-crosslinked chitosan with high amino group contents

Author:
Kim, Ung-Jin, Lee, Yeong Ro, Kang, Tong Ho, Choi, Joon Weon, Kimura, Satoshi, Wada, Masahisa
Source:
Carbohydrate polymers 2017 v.163 pp. 34-42
ISSN:
0144-8617
Subject:
adsorption, aldehydes, bovine serum albumin, cellulose, chitosan, crosslinking, cytotoxicity, oxidation, pH, schiff bases, solubilization
Abstract:
Crosslinked chitosan was prepared by Schiff base formation between the aldehyde groups of dialdehyde cellulose (DAC) and the amino groups of chitosan and a subsequent reduction. DAC was obtained through periodate oxidation of cellulose and solubilization in hot water at 100°C for 1h. Three grades of DAC-crosslinked chitosan were prepared by adding various amounts DAC. The degrees of crosslinking as determined by amino group content were 3.8, 8.3, and 12.1%, respectively. DAC-crosslinked chitosan showed higher stability in the pH 2–9 range and no cytotoxicity was identified over the course of a 21-day long-term stability test. Also, DAC-crosslinked chitosan showed remarkably high bovine serum albumin (BSA) adsorption capacity at pH 5.5 as a result of the increased amino group content, due to the reaction between DAC and chitosan molecular chains occurring at multiple points even though DAC-crosslinked chitosan showed a lower degree of crosslinking.
Agid:
5617952