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Acylation of soluble polysaccharides in a biphasic system catalyzed by a CE2 acetyl esterase

Author:
Kanelli, Maria, Topakas, Evangelos
Source:
Carbohydrate polymers 2017 v.163 pp. 208-215
ISSN:
0144-8617
Subject:
Clostridium thermocellum, Fourier transform infrared spectroscopy, acylation, beta-glucans, biodegradability, disaccharides, esterases, esters, monosaccharides, screening, transesterification
Abstract:
Within the last decade, acylated polysaccharides have drawn attention, since they find applications in numerous fields as biocompatible and biodegradable amphiphilic compounds. The ability of a CE2 acetyl esterase from Clostridium thermocellum to catalyze acyl transfer to β-glucan and manno-polysaccharides of significant interest was investigated. Initially, screening tests were conducted on aldohexose monosaccharides and disaccharides, exploiting the enzyme’s strict regioselectivity at O-6 position. Modified monosaccharides acquired acylation yields from 11 up to 65%, showing preference for small chain acyl donors, while disaccharides exhibited conversion yields from 23 up to 58%, with preference to monoacylation. The transesterification reactions were carried out in two-phase mixtures consisted of water/vinyl esters. Acylation of polysaccharides were confirmed by TLC and FT-IR, while the degree of acylation was determined via an indirect method, estimating a range of acylation from 0.022 to 1.083mmolacylgroup*gpolysaccharide−1 depending on the structure and composition of the target polysaccharide.
Agid:
5617957