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Biodegradation of the benzo[a]pyrene-contaminated sediment of the Jiaozhou Bay wetland using Pseudomonas sp. immobilization

Jin, Xin, Tian, Weijun, Liu, Qing, Qiao, Kaili, Zhao, Jing, Gong, Xiaoxi
Marine pollution bulletin 2017 v.117 no.1-2 pp. 283-290
Pseudomonas, adsorption, bacteria, benzo(a)pyrene, biodegradation, chitosan, coal, immobilized cells, kinetics, sediments, wetlands
To remove benzo[a]pyrene (BaP) that has accumulated in the Jiaozhou Bay wetland sediment, two strains (JB1 and JB2) were selected from the BaP-contaminated the wetland sediment and immobilized in coal cinder and chitosan beads using entrapping and surface adsorption methods. Biodegradation of BaP in sediment was carried out in pots. The results showed that, supported by the coal cinder and chitosan beads, 71.9, 65.5, 58.9 and 66.1% of the BaP in the immobilized cells was degraded after 40d. These percentages were clearly higher than the 47.7% that degraded from free cells. Kinetic analysis indicated that the immobilized gel-beads might remove BaP by multiple control steps. Compared to the chitosan, coal cinder-entrapping beads exhibited a higher removal rate for BaP; however, the degradation rates from coal cinder- and chitosan-surface adsorption beads were almost the same. This result indicates that in addition to the BaP-degrading bacteria, carrier materials and immobilizing methods play an important role in determining the success of a biodegradation strategy.