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Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain
- Li, Xiaowei, Tzeng, Stephany Y., Liu, Xiaoyan, Tammia, Markus, Cheng, Yu-Hao, Rolfe, Andrew, Sun, Dong, Zhang, Ning, Green, Jordan J., Wen, Xuejun, Mao, Hai-Quan
- Biomaterials 2016 v.84 pp. 157-166
- biodegradability, brain, humans, hyaluronic acid, hydrocolloids, models, neurons, rats, stem cells, tissue repair, tissue transplantation, transcription (genetics), transcription factors, transcriptional activation, transfection
- Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue-derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy.