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A two-column flash chromatography approach to pyoverdin production from Pseudomonas putida GB1
- Duckworth, Owen W., Markarian, Dawn S., Parker, Dorothy L., Harrington, James M.
- Journal of microbiological methods 2017 v.135 pp. 11-13
- Pseudomonas putida, absorption, chromatography, siderophores
- Our knowledge of the biological and environmental reactivity of siderophores is limited by the difficulty and cost of obtaining reasonable quantities by purification or synthesis. In this note, we describe a modified procedure for the low-cost, mg-scale purification of pyoverdin-type siderophores using a dual-flash chromatography (reverse-phase absorption and size exclusion) approach.