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Optimized use of hemicellulose within a biorefinery for processing high value-added xylooligosaccharides

Álvarez, Cristina, González, Alberto, Negro, Maria José, Ballesteros, Ignacio, Oliva, José Miguel, Sáez, Felicia
Industrial crops and products 2017 v.99 pp. 41-48
beta-glucosidase, biorefining, cellulose, cleaning, endo-1,4-beta-xylanase, enzymatic hydrolysis, hydrolysis, ion exchange resins, lignin, lignocellulose, polymerization, prebiotics, raw materials, value added, wheat straw, xylan, xylooligosaccharides
Wheat straw was used to produce xylooligosaccharides (XOS) for its prebiotic effect. Steam explosion (SE) pre-treatment has been used to separate the main components of lignocellulosic material: cellulose, hemicellulose and lignin. SE conditions used gave a cellulose recovery as solid fraction (91%) and a xylan recovered in the liquid fraction (LF) (36%). Previously to XOS production by enzymatic hydrolysis of xylan, the LF was subjected to a cleaning step with ion exchange resin to remove soluble inhibitors. The commercial enzyme endo-1,4-β-xylanase was used individually and in combination with β-glucosidase to produce compounds with a low degree of polymerization (DP2-DP6). The process achieved a conversion rate of 91% to XOS with 5h of hydrolysis supplementation, whatever generated 8.9g of XOS per 100g of raw material.