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Selective enrichment of dairy phospholipids in a buttermilk substrate through investigation of enzymatic hydrolysis of milk proteins in conjunction with ultrafiltration

Barry, Kate M., Dinan, Timothy G., Kelly, Philip M.
International dairy journal 2017 v.68 pp. 80-87
active ingredients, buttermilk, enzymatic hydrolysis, hydrolysis, lipid content, milk proteins, peptides, phospholipids, ultrafiltration
Extensive enzymatic hydrolysis of milk proteins in reconstituted buttermilk powder was combined with ultrafiltration to generate a phospholipid (PL) enriched fraction with maximum permeation of hydrolysed peptides. Buttermilk, naturally high in PLs, is the ideal substrate for enrichment of these bio- and techno-functionally active compounds. A 7.8 fold increase in PL was achieved in the 50 kDa retentate; 6.16±0.02% total PL compared with 0.79±0.01% in the starting substrate, an increase considerably greater than previously reported. Total lipid content (% dry matter) increased 6.3 fold in the retentate, 43.43±0.61%, from the starting substrate, 6.84±0.17%. This combined strategic approach enabled maximum enrichment of PLs with no transmission of lipid material into the permeate, 0.09±0.02% total lipid, and non-detectable levels of PLs recovered in the permeate, 0.00±0.01% total PL.