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A report on extensive lateral genetic reciprocation between arsenic resistant Bacillus subtilis and Bacillus pumilus strains analyzed using RAPD-PCR

Author:
Khowal, Sapna, Siddiqui, Md. Zulquarnain, Ali, Shadab, Khan, Mohd. Taha, Khan, Mather Ali, Naqvi, Samar Husain, Wajid, Saima
Source:
Molecular phylogenetics and evolution 2017 v.107 pp. 443-454
ISSN:
1055-7903
Subject:
Bacillus pumilus, Bacillus sonorensis, Bacillus subtilis, DNA, arsenic, arsenites, bacteria, genetic markers, genetic similarity, horizontal gene transfer, metal tolerance, nucleotide sequences, phylogeny, random amplified polymorphic DNA technique, ribosomal RNA, soil sampling
Abstract:
The study involves isolation of arsenic resistant bacteria from soil samples. The characterization of bacteria isolates was based on 16S rRNA gene sequences. The phylogenetic consanguinity among isolates was studied employing rpoB and gltX gene sequence. RAPD-PCR technique was used to analyze genetic similarity between arsenic resistant isolates. In accordance with the results Bacillus subtilis and Bacillus pumilus strains may exhibit extensive horizontal gene transfer. Arsenic resistant potency in Bacillus sonorensis and high arsenite tolerance in Bacillus pumilus strains was identified. The RAPD-PCR primer OPO-02 amplified a 0.5kb DNA band specific to B. pumilus 3ZZZ strain and 0.75kb DNA band specific to B. subtilis 3PP. These unique DNA bands may have potential use as SCAR (Sequenced Characterized Amplified Region) molecular markers for identification of arsenic resistant B. pumilus and B. subtilis strains.