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Ultrasound-assisted ionic liquid-based micellar extraction combined with microcrystalline cellulose as sorbent in dispersive microextraction for the determination of phenolic compounds in propolis

Cao, Jun, Peng, Li-Qing, Du, Li-Jing, Zhang, Qi-Dong, Xu, Jing-Jing
Analytica chimica acta 2017
analytical chemistry, bromine, cellulose, detection limit, ionic liquids, liquid chromatography, pH, phenolic compounds, propolis, solid phase microextraction, tandem mass spectrometry, ultrasonic treatment, ultrasonics
An ionic liquid-(IL) based micellar extraction combined with microcrystalline cellulose- (MCC) assisted dispersive micro solid-phase extraction method was developed to extract phenolic compounds from propolis. A total of 20 target compounds were identified by ultra-high- performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. The main extraction parameters were optimized and included the ultrasonic power, ultrasonic time, sample pH, type of IL, the concentration of [C12mim]Br, extraction time, concentration of MCC, type of sorbent and type of elution solvents. Under the optimum conditions, the proposed method exhibited good linearities (r2 ≥ 0.999) for all plant phenolic compounds with the lower limits of detection in the range of 0.21–0.41 ng/mL. The recoveries ranged from 82.74% to 97.88% for pinocembrin, chrysin and galangin. Compared with conventional solvent extraction, the present method was simpler and more efficient and required less organic solvent and a shorter extraction time. Finally, the methodology was successfully used for the extraction and enrichment of phenolic compounds in propolis.