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Genomic sequencing of a strain of Acinetobacter baumannii and potential mechanisms to antibiotics resistance

Zhao, Lei, Li, Hongru, Zhu, Ziwen, Wakefield, Mark R., Fang, Yujiang, Ye, Ying
Infection, genetics, and evolution 2017 v.50 pp. 20-24
ABC transporters, Acinetobacter baumannii, DNA, DNA repair, antibiotic resistance, beta-lactamase, biochemical pathways, cephalosporins, genes, penicillins, sequence analysis, single nucleotide polymorphism, transposition (genetics), China
Acinetobacter baumannii has been becoming a great challenge to clinicians due to their resistance to almost all available antibiotics. In this study, we sequenced the genome from a multiple antibiotics resistant Acinetobacter baumannii stain which was named A. baumannii-1isolated from China by SMRT sequencing technology to explore its potential mechanisms to antibiotic resistance. We found that several mechanisms might contribute to the antibiotic resistance of Acinetobacter baumannii. Specifically, we found that SNP in genes associated with nucleotide excision repair and ABC transporter might contribute to its resistance to multiple antibiotics; we also found that specific genes associated with bacterial DNA integration and recombination, DNA-mediated transposition and response to antibiotics might contribute to its resistance to multiple antibiotics; Furthermore, specific genes associated with penicillin and cephalosporin biosynthetic pathway and specific genes associated with CHDL and MBL β-lactamase genes might contribute to its resistance to multiple antibiotics. Thus, the detailed mechanisms by which Acinetobacter baumannii show extensive resistance to multiple antibiotics are very complicated. Such a study might be helpful to develop new strategies to control Acinetobacter baumannii infection.