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Aurora Kinase B Phosphorylates Histone H3.3 at Serine 31 during Mitosis in Mammalian Cells

Author:
Li, Miao, Dong, Qiang, Zhu, Bing
Source:
Journal of Molecular Biology 2017
ISSN:
0022-2836
Subject:
chromosome segregation, enzyme activity, enzyme inhibitors, histones, humans, mitosis, non-specific serine/threonine protein kinase, phosphorylation, screening, serine, small interfering RNA
Abstract:
Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro.
Agid:
5633780