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The influence of pregnancy and plane of nutrition during pregnancy on pancreatic digestive enzymes and insulin-containing cell cluster morphology in beef cows

Author:
Awda, B.J., Wood, K.M., Keomanivong, F.E., Borowicz, P.P., McBride, B.W., Miller, S.P., Fitzsimmons, C.J., Swanson, K.C.
Source:
Canadian journal of animal science 2016 v.97 no.1 pp. 145-153
ISSN:
1918-1825
Subject:
alpha-amylase, beef cows, energy, feeding level, pancreas, parturition, pregnancy, trypsin
Abstract:
To determine effects of pregnancy (experiment 1) and plane of nutrition during pregnancy (experiment 2) on pancreatic digestive enzymes and morphology of insulin-containing cell clusters, beef cows were slaughtered, and the pancreas collected 4 wk before parturition (if pregnant or similar time on feed for nonpregnant cows). In experiment 1, mature, nonlactating cows [717 ± 70 kg; nine pregnant (PREG) and nine nonpregnant (OPEN)] were used. In experiment 2, nonlactating, pregnant cows (639 ± 68 kg) were fed at 85% (n = 11; LOW) or 140% (n = 11; HIGH) of net energy (NE) requirements. Concentration (U g⁻¹) and content (U kg⁻¹ BW) of α-amylase and trypsin activities were greater (P ≤ 0.05) in OPEN than PREG cows. Pregnant cows had greater (P ≤ 0.03) insulin-cell cluster size and proportion of large insulin-containing cell clusters than OPEN cows. Concentration and content of α-amylase activity were greater (P ≤ 0.04) in HIGH than LOW cows. These data indicate that pregnancy status (PREG vs. OPEN) impacts pancreatic exocrine and endocrine functions and that plane of nutrition (85% vs. 140% of NE requirements) of pregnant cows may not greatly impact pancreatic exocrine and endocrine functions.
Agid:
5635200