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Encapsulation of Gadolinium Oxide Nanoparticle (Gd2O3) Contrasting Agents in PAMAM Dendrimer Templates for Enhanced Magnetic Resonance Imaging in Vivo

Author:
Mekuria, Shewaye Lakew, Debele, Tilahun Ayane, Tsai, Hsieh-Chih
Source:
ACS Applied Materials & Interfaces 2017 v.9 no.8 pp. 6782-6795
ISSN:
1944-8252
Subject:
biocompatibility, bladder, encapsulation, gadolinium, intestines, kidneys, liver, macrophages, magnetic resonance imaging, nanoparticles, polyethylene glycol, spleen
Abstract:
There has been growing interest in the research of nanomaterials for biomedical applications in recent decades. Herein, a simple approach to synthesize the G4.5-Gd₂O₃-poly(ethylene glycol) (G4.5-Gd₂O₃-PEG) nanoparticles (NPs) that demonstrate potential as dual (T₁ and T₂) contrasting agents in magnetic resonance imaging (MRI) has been reported in this study. Compared to the clinically popular Gd-DTPA contrasting agents, G4.5-Gd₂O₃-PEG NPs exhibited a longer longitudinal relaxation time (T₁) and better biocompatibility when incubated with macrophage cell line RAW264.7 in vitro. Furthermore, the longitudinal relaxivity (r₁) of G4.5-Gd₂O₃-PEG NPs was 53.9 s–¹ mM–¹ at 7T, which is equivalent to 4.8 times greater than to the Gd-DTPA contrasting agents. An in vivo T₁-weighted MRI results revealed that G4.5-Gd₂O₃-PEG NPs significantly enhanced signals in the intestines, kidney, liver, bladder, and spleen. In addition, the T₂-weighted MRI results revealed darker contrast in the kidney, which proves that G4.5-Gd₂O₃-PEG NPs can be exploited as T₁ and T₂ contrasting agents. In summary, these findings suggest that the G4.5-Gd₂O₃-PEG NPs synthesized by an alternative approach can be used as dual MRI contrasting agents.
Agid:
5635707