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Studies on the biocontrol mechanisms of Pseudomonas graminis strain CPA-7 against food-borne pathogens in vitro and on fresh-cut melon

Author:
Collazo, Cyrelys, Abadias, Maribel, Aguiló-Aguayo, Ingrid, Alegre, Isabel, Chenoll, Empar, Viñas, Inmaculada
Source:
Lebensmittel-Wissenschaft + [i.e. und] Technologie 2017 v.85 pp. 301-308
ISSN:
0023-6438
Subject:
Escherichia coli O157, Listeria monocytogenes, Pseudomonas graminis, Salmonella enterica, alginates, antagonists, bacteria, biofilm, biological control, biological control agents, biosurfactants, carboxylic acids, culture media, food pathogens, fresh-cut produce, hydrophobicity, in vitro studies, mechanism of action, melons, metabolites, nutrient content, polystyrenes, proteolysis
Abstract:
The present study was aimed at gaining insight into the mode of action of the antagonistic bacteria Pseudomonas graminis CPA-7, which has been previously identified as an effective biocontrol agent against Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 on fresh-cut fruit. In vitro experiments did not reveal any antimicrobial or proteolytic activity on solid media or any biosurfactant activity on hydrophobic surfaces. Metabolites produced by CPA-7 in two different culture media and on 'Galia' melon were unable to inhibit L. monocytogenes populations on 'Galia' melon plugs at 25 °C or 5 °C. In contrast, at 25 °C the population of this pathogen on 'Galia' plugs was reduced by 2.1 and 3.3 log-units when co-inoculated with the antagonist in water, after 24 and 48 h, respectively. CPA-7 did not form biofilms after 72 h at 25 °C (OD = 0.03) or at 30 °C (OD = 0.01) on polystyrene plates and the production of alginate was close to the negative control. Studies of nutritional profiles showed high overlap (NOI > 0.9) between CPA-7 and E. coli O157:H7 regarding the use of carboxylic acids. This functional group could also contain putative targets for competiveness between CPA-7 and S. enterica, although overlapping was not restrictive enough (NOI = 0.83).
Agid:
5636957