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Preparation of monoclonal antibody and development of an indirect competitive enzyme-linked immunosorbent assay for ornidazole detection
- Zhao, Liang, Li, Jiaying, Li, Ye, Wang, Tiangang, Jin, Xiaolu, Wang, Kai, Rahman, Ebeydulla, Xing, Yuan, Ji, Baoping, Zhou, Feng
- Food chemistry 2017 v.229 pp. 439-444
- bovine serum albumin, chickens, cross reaction, detection limit, enzyme-linked immunosorbent assay, haptens, honey, liver, mice, monoclonal antibodies, rapid methods
- A monoclonal antibody (mAb) and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ornidazole (ONZ) detection were developed. ONZ was conjugated with cationic bovine serum albumin as a hapten to generate the artificial immunogens and coating antigens. BALB/c mice were immunized, and mAbs were obtained. The competitive inhibition curve of ic-ELISA was y=0.0438x2−0.2101x+0.2925, with R2=0.9941. The 50% inhibition concentration, the limit of detection, and limit of quality for ONZ were 0.15, 0.01, and 0.05µg/kg, respectively. The cross-reactivity of the mAbs to secnidazole was 0.33%. The recoveries were from 89.18% to 101.63% and the coefficient of variation was less than 7.15% in chicken, chicken liver, and honey samples, all of which had ONZ concentrations of 0.05 and 0.1μg/kg. Results showed that the ic-ELISA based on mAb could be used for the rapid detection for ONZ.