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Clostridium difficile in faeces from healthy dogs and dogs with diarrhea

Author:
Wetterwik, Karl-Johan, Trowald-Wigh, Gunilla, Fernström, Lise-Lotte, Krovacek, Karel
Source:
Acta veterinaria scandinavica 2013 v.55 no.1 pp. 23
ISSN:
1751-0147
Subject:
Clostridium difficile, diarrhea, dog diseases, dogs, feces, genes, metronidazole, polymerase chain reaction, toxins, Sweden
Abstract:
BACKGROUND: This study was conducted to evaluate the faecal occurrence and characterization of Clostridium difficile in clinically healthy dogs (N = 50) and in dogs with diarrhea (N = 20) in the Stockholm-Uppsala region of Sweden. FINDINGS: Clostridium difficile was isolated from 2/50 healthy dogs and from 2/20 diarrheic dogs. Isolates from healthy dogs were negative for toxin A and B and for the tcdA and tcdB genes. Both isolates from diarrheic dogs were positive for toxin B and for the tcdA and tcdB genes. The C. difficile isolates from healthy dogs had PCR ribotype 009 (SE-type 6) and 010 (SE-type 3) whereas both isolates from dogs with diarrhoea had the toxigenic ribotype 014 (SE-type 21). One of the isolates from healthy dogs was initially resistant to metronidazole. CONCLUSIONS: This study revealed presence of toxigenic C. difficile in faecal samples of diarrheic dogs and low number of non- toxigenic isolates in healthy dogs from Uppsala-Stockholm region in Sweden. However, more comprehensive studies are warranted to investigate the role of C. difficile in gastrointestinal disease in dogs.
Agid:
564106