U.S. flag

An official website of the United States government

PubAg

Main content area

GADD45ß, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens

Author:
Zhang, Xinheng, Yan, Zhuanqiang, Li, Xinjian, Lin, Wencheng, Dai, Zhenkai, Yan, Yiming, Lu, Piaopiao, Chen, Weiguo, Zhang, Huanmin, Chen, Feng, Ma, Jingyum, Xie, Qingmei
Source:
Oncotarget 2016 v.7 no.42 pp. 68883-68893
ISSN:
1949-2553
Subject:
Avian leukosis virus, antineoplastic activity, chickens, gene expression, gene expression regulation, genes, genetic markers, genetic resistance, immunosuppression, liver, neoplasms, sequence analysis, signal transduction, virus replication
Abstract:
Avian leukosis virus subgroup J (ALV-J) is a retrovirus that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that confer genetic resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens. RNA-sequencing of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens in contrast to the susceptible ones. We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45ß inhibited ALV-J replication. GADD45ß could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.
Agid:
5642514
Handle:
10113/5642514