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GADD45ß, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens

Zhang, Xinheng, Yan, Zhuanqiang, Li, Xinjian, Lin, Wencheng, Dai, Zhenkai, Yan, Yiming, Lu, Piaopiao, Chen, Weiguo, Zhang, Huanmin, Chen, Feng, Ma, Jingyum, Xie, Qingmei
Oncotarget 2016 v.7 no.42 pp. 68883-68893
Avian leukosis virus, antineoplastic activity, chickens, gene expression, gene expression regulation, genes, genetic markers, genetic resistance, immunosuppression, liver, neoplasms, sequence analysis, signal transduction, virus replication
Avian leukosis virus subgroup J (ALV-J) is a retrovirus that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that confer genetic resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens. RNA-sequencing of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens in contrast to the susceptible ones. We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45ß inhibited ALV-J replication. GADD45ß could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.