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Trehalose combined with sodium dichloroisocyanurate reduces microbial load in the vase solution and improves the quality of ‘Grand Gala’ cut rose flowers

Worarad, K., Sanguanpuag, K., Wongs-Aree, C., Buanong, M.
Acta horticulturae 2016 no.1131 pp. 73-80
corolla, ethylene production, fluorescence, microbial growth, microbial load, microorganisms, scanning electron microscopy, sodium dichloroisocyanurate, stems, sugar content, trehalose, vase life, water uptake, xylem
The effects of a combination of trehalose and sodium dichloroisocyanurate (DICA) on reducing the growth of microorganisms in the vase solution and improving the quality of 'Grand Gala' cut rose flowers was investigated. Flower stems were held in distilled water (control; DW), Floralife®, 2% trehalose (Tre) and 2% trehalose + 50 mg L-1 DICA (Tre + DICA) for 7 d in an observation room (21±2°C, 70-80% RH, cool-white fluorescence lights for 12 h d-1). The results showed that treatment of Tre + DICA was the most effective for inhibiting microbial growth, followed by Floralife® solution which delayed the increase in microbial content in the vase solution. In contrast, flowers held in DW and Tre had the greatest number of microorganisms in the vase solution. This was related to the microbial content observed in the xylem on days 0 and 6 by scanning electron microscopy (SEM). Microbial growth was almost undetectable in the Tre + DICA treatment while 'Grand Gala' flowers held in Tre and the control had significant microbial content in the xylem in day 6. Additionally, cut rose flowers held in the Floralife® solution and Tre + DICA increased the water uptake and had the longest vase life of 7.8 and 7.6 days, respectively, while, the control flowers showed the shortest vase life of 4.9 days. Treatments of Tre + DICA and Tre had significantly higher total sugar content in petals than others. However, there were no significant differences in fresh weight and ethylene production among all treatments.