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Bead-beating artefacts in the Bacteroidetes to Firmicutes ratio of the human stool metagenome

Author:
Vebø, Heidi C., Karlsson, Magdalena Kauczynska, Avershina, Ekaterina, Finnby, Lene, Rudi, Knut
Source:
Journal of microbiological methods 2016 v.129 pp. 78-80
ISSN:
0167-7012
Subject:
Bacteroidetes, Firmicutes, feces, metagenomics
Abstract:
We evaluated bead-beating cell-lysis in analysing the human stool metagenome, since this is a key step. We observed that two different bead-beating instruments from the same producer gave a three-fold difference in the Bacteroidetes to Firmicutes ratio. This illustrates that bead-beating can have a major impact on downstream metagenome analyses.
Agid:
5645197