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Aberrant expression of liver microRNA in chickens infected with subgroup J avian leukosis virus

Author:
Li, Hongmei, Ji, Jun, Xie, Qingmei, Shang, Huiqin, Zhang, Huanmin, Xin, Xuegang, Chen, Feng, Sun, Baoli, Xue, Chunyi, Ma, Jingyun, Bi, Yingzuo
Source:
Virus Research 2012 v.169 pp. 268
ISSN:
0168-1702
Subject:
Avian leukosis virus, carcinogenicity, chickens, epigenetics, gene expression, genes, liver, microRNA, microarray technology, neoplasms, poultry industry, receptors, United States
Abstract:
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus primarily causing myeloid leukosis (ML) in broilers. Although ALV is well under control in a few countries including the USA, poultry industry in many parts of the world continues suffering from serious economic loss due to sporadic or widespread ALV infection, especially ALV-J infection. ALV-J infection of chickens is reportedly mediated by a cellular receptor. So far, however, no genetic variant of the receptor gene that confers resistance to ALV-J has been identified. To advance our understanding on epigenetic factors that are involved in the event of ALV-J infection, we examined the expression of miRNAs in livers of 10-week-old chickens uninfected or infected with ALV-J by miRNA microarray analysis. Our data showed there were 12 miRNAs differentially expressed in liver between the uninfected and infected groups (P < 0.01). Of which, the expressions of seven miRNAs (gga-mir-221, gga-mir-222, gga-mir-1456, gga-mir-1704, gga-mir-1777, gga-mir-1790, and gga-mir-2127,) were upregulated by ALV-J infection and may be involved in oncogenicity. The other five miRNAs (gga-let-7b, gga-let-7i, gga-mir-125b, gga-mir-375, and gga-mir-458) were significantly downregulated. The downregulated miRNAs may play important roles in tumor suppression. This finding paves the way for further exploration of epigenetic influence on tumorigenicity upon ALV-J infection.
Agid:
56452
Handle:
10113/56452