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Highly oxygenated lanostane-type triterpenoids and their bioactivity from the fruiting body of Ganoderma gibbosum

Pu, De-Bing, Zheng, Xi, Gao, Jun-Bo, Zhang, Xing-Jie, Qi, Yan, Li, Xiao-Si, Wang, Yong-Mei, Li, Xiao-Nian, Li, Xiao-Li, Wan, Chun-Ping, Xiao, Wei-Lie
Fitoterapia 2017 v.119 pp. 1-7
Ganoderma, T-lymphocytes, X-ray diffraction, acids, anti-inflammatory activity, fruiting bodies, immunostimulants, lipopolysaccharides, lymphocyte proliferation, macrophages, mice, nuclear magnetic resonance spectroscopy, triterpenoids
Eight new highly oxygenated lanostane triterpenes, gibbosic acids A–H (1–8), along with three known ones (9–11), were isolated from the fruiting body of Ganoderma gibbosum. The structures of new isolates were assigned by NMR and HRESIMS experiments. The absolute configurations of 1 were further confirmed by single crystal X-ray diffraction data and computational ECD methods. Immunoregulatory effect and anti-inflammatory activities of these compounds were screened in murine lymphocyte proliferation assay and in lipopolysaccharide (LPS)-stimulated RAW-264.7 macrophages, respectively. Compound 2 exhibited immunostimulatory effect both in lymphocyte proliferation assay without any induction and ConA-induced mitogenic activity of T-lymphocyte, and the proportion of lymphocyte proliferation at the concentration of 0.1μM are 20.01% and 21.40%, respectively.