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Rhizobium sphaerophysae sp. nov., a novel species isolated from root nodules of Sphaerophysa salsula in China

Author:
Xu, Lin, Shi, Jian Feng, Zhao, Peng, Chen, Wei Min, Qin, Wei, Tang, Ming, Wei, Ge Hong
Source:
Antonie van Leeuwenhoek 2011 v.99 no.4 pp. 845-854
ISSN:
0003-6072
Subject:
Rhizobium giardinii, Sphaerophysa salsula, new species, nucleotide sequences, pH, phylogeny, ribosomal RNA, root nodules, China
Abstract:
Four gram-negative, aerobic, motile, non-spore, forming rods with a wide pH and temperature range for growth (pH 7.0-11.0, optimum pH 8.0; 20-45°C, optimum 28°C) strains were isolated from root nodules of Sphaerophysa salsula and characterized by means of a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the four strains formed a new lineage related to the genus Rhizobium and the sequence similarities between the isolate and the most related type strain Rhizobium giardinii was 96.5%. These strains also formed a distinctive group from the reference strains for defined Rhizobium species based on housekeeping gene sequences (atpD and recA), BOX-PCR fingerprinting, phenotypic features and symbiotic properties. The representative strain CCNWGS0238T has DNA-DNA relatedness of less than 33.4% with the most closely related species R. giardinii. It is therefore proposed as a new species, Rhizobium sphaerophysae sp. nov., with isolate CCNWGS0238T (=ACCC17498T = HAMBI3074T) as the type strain.
Agid:
565000