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Multi-residue determination of 210 drugs in pork by ultra-high-performance liquid chromatography–tandem mass spectrometry A

Yin, Zhiqiang, Chai, Tingting, Mu, Pengqian, Xu, Nana, Song, Yue, Wang, Xinlu, Jia, Qi, Qiu, Jing
Journal of chromatography 2016 v.1463 pp. 49-59
acetonitrile, adrenal cortex hormones, alkaloids, aminoglycosides, animal tissues, antifungal agents, antiviral agents, beta-adrenergic agonists, beta-lactams, defatting, food safety, imidazoles, insecticides, liquid chromatography, macrolides, monitoring, multiresidue analysis, nonsteroidal anti-inflammatory agents, phenothiazine, piperidines, pork, quinolones, solvents, statistical analysis, sulfonamides, tandem mass spectrometry, tetracyclines, veterinary drugs
This paper presents a multi-residue analytical method for 210 drugs in pork using ultra-high-performance liquid chromatography-Q-Trap tandem mass spectrometry (UPLC–MS/MS) within 20min via positive ESI in scheduled multi-reaction monitoring (MRM) mode. The 210 drugs, belonging to 21 different chemical classes, included macrolides, sulfonamides, tetracyclines, β-lactams, β-agonists, aminoglycosides, antiviral drugs, glycosides, phenothiazine, protein anabolic hormones, non-steroidal anti-inflammatory drugs (NSAIDs), quinolones, antifungal drugs, corticosteroids, imidazoles, piperidines, piperazidines, insecticides, amides, alkaloids and others. A rapid and simple preparation method was applied to process the animal tissues, including solvent extraction with an acetonitrile/water mixture (80/20, v/v), defatting and clean-up processes. The recoveries ranged from 52% to 130% with relative standard deviations (RSDs)<20% for spiked concentrations of 10, 50 and 250μg/kg. More than 90% of the analytes achieved low limits of quantification (LOQs)<10μg/kg. The decision limit (CCα), detection capability (CCβ) values were in the range of 2–502μg/kg and 4–505μg/kg, respectively. This method is significant for food safety monitoring and controlling veterinary drug use.