Main content area

Effects of cultured Cordyceps mycelia polysaccharide A on tumor neurosis factor-α induced hepatocyte injury with mitochondrial abnormality

Tang, Huiling, Wei, Weikun, Wang, Wang, Zha, Zhengqi, Li, Ting, Zhang, Zhijie, Luo, Chen, Yin, Hongping, Huang, Fengjie, Wang, Ying
Carbohydrate polymers 2017 v.163 pp. 43-53
Cordyceps, Ophiocordyceps sinensis, apoptosis, caspase-3, caspase-9, chromatography, ion exchange, membrane potential, mitochondria, molecular weight, mycelium, peroxisome proliferator-activated receptors, polysaccharides, protective effect, tumor necrosis factor-alpha
Cordyceps sinensis mycelia polysaccharide A (CPS-A), was isolated from cultured Cordyceps mycelia by 65% alcohol extraction and ion-exchange column chromatography. The molecular weight of CPS-A was 1.2×104Da and the backbone was mainly composed of (1→2)-linked β-d-mannopyranose, (1→2,4)-linked β-d-mannopyranose and (1→4)-linked α-d-glucopyranose with terminal β-d-mannopyranose and α-d-glucopyranose residues. CPS-A played a protective role against TNF-α induced mitochondria injury in L02 cells via up-regulation of mitofusin 2, peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), and membrane potential. CPS-A also played a protective role against TNF-α induced L02 cells apoptosis via up-regulation of Bcl-2 and down-regulation of Bid, Bax, cleaved caspase-3, cleaved caspase-9 and ROS production. Moreover, CPS-A attenuated both the normal expression and overexpression of TNF-α receptor 1 (TNFR1) induced by TNF-α administration. In conclusion, CPS-A was involved in TNF-α induced mitochondria abnormality via TNFR1/ROS/Mfn2 pathway.