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Formation of low molecular weight oligomers from chitin and chitosan stimulated by plasma-assisted processes

Author:
Vasilieva, T., Sigarev, A., Kosyakov, D., Ul’yanovskii, N., Anikeenko, E., Chuhchin, D., Ladesov, A., Hein, Aung Myat, Miasnikov, V.
Source:
Carbohydrate polymers 2017 v.163 pp. 54-61
ISSN:
0144-8617
Subject:
active oxygen species, chitin, chitooligosaccharides, chitosan, fungi, growth retardation, hydrolysis, minimum inhibitory concentration, molecular weight, polymers, powders, temperature
Abstract:
The controlled degradation of solid powders of chitin and chitosan stimulated by electron-beam plasma (EBP) was experimentally studied. Crab shell chitin and chitosan were used as original substances. The non-equilibrium low temperature EBP was generated by injecting an electron beam into a gaseous medium. Chitooligosaccharides with Mw=800–2000Da and polydispersion 1.5–2.5 were formed due to the EBP-treatment of chitin and chitosan. The β-1,4 glycosidic bounds in original polymers degrade under the action of active oxygen species produced in the EBP. Low molecular weight products of chitosan inhibited the growth of various yeast-like and filamentous fungi at minimum inhibitory concentration 500mcg/ml. By optimizing the treatment conditions and using special techniques of reaction volume formation the 95% yield of chitooligosaccharides was obtained after 2min whereas the conventional chemical hydrolysis usually takes several days. The EBP-stimulated hydrolysis is promising for effective polysaccharides degradation and can be competitive with traditional technologies.
Agid:
5651066