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Endocrine-related genes are altered by antibacterial agent triclosan in Chironomus riparius aquatic larvae

Martínez-Paz, Pedro, Morales, Mónica, Urien, Josune, Morcillo, Gloria, Martínez-Guitarte, José Luis
Ecotoxicology and environmental safety 2017 v.140 pp. 185-190
Chironomus riparius, antibiotics, aquatic ecosystems, ecdysone receptor, gene expression regulation, genes, heat shock proteins, hormones, insects, larvae, mechanism of action, quantitative polymerase chain reaction, reverse transcriptase polymerase chain reaction, toxicology, transcription (genetics)
Triclosan (TCS) is an antibacterial agent widely used in personal care and consumer products and commonly detected in aquatic ecosystems. In the present study, the effects of TCS on endocrine-related genes of Chironomus riparius aquatic larvae, a reference organism in aquatic toxicology, were evaluated. Twenty-four-hour in vivo exposures at 10µg/L, 100µg/L, and 1000µg/L TCS revealed that this xenobiotic was able to alter the transcriptional activity of ecdysone receptor gene (EcR), the ultraspiracle gene (usp), the estrogen-related receptor gene (ERR), and the E74 early ecdysone-inducible gene, as measured by real-time RT-PCR. Moreover, the hsp70 gene, a heat shock protein gene, was upregulated after exposure to TCS. The results of the present work provide the first evidence of the potential disruptive effects of TCS in endocrine-related genes suggesting a mode of action that mimics ecdysteroid hormones in insects.