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Newcastle disease virus-like particles induce DC maturation through TLR4/NF-κB pathway and facilitate DC migration by CCR7-CCL19/CCL21 axis

Qian, Jing, Xu, Xiaohong, Ding, Jiaxin, Yin, Renfu, Sun, Yixue, Xue, Cong, Wang, Jianzhong, Ding, Chan, Yu, Shengqing, Liu, Xiufan, Hu, Shunlin, Wang, Chunfeng, Cong, Yanlong, Ding, Zhuang
Veterinary microbiology 2017 v.203 pp. 158-166
CCR7 receptor, CD4-positive T-lymphocytes, Newcastle disease, chemical elements, chickens, dendritic cells, immune response, innate immunity, interferon-gamma, interleukin-4, mice, vaccines, virus-like particles
Newcastle disease virus-like particles (NDV VLPs) are a potential candidate vaccine, as shown by eliciting specific immune response against NDV in mice and chickens. Activation of dendritic cells (DCs) is critical to initiate immune response. However, the mechanism of how NDV VLPs induce DC maturation and migration remains elusive. In this study, we found that NDV VLPs are efficient in DC activation by up-regulating surface MHC II and costimulatory molecules, and proinflammatory cytokines through the TLR4/NF-κB pathway. Furthermore, NDV VLPs elevated CCR7 expression on DCs, resulting in DC migration towards CCL19/CCL21 both in vitro and ex vivo. As a consequence of DC maturation and migration, CD4+ T cells were also activated in vivo, demonstrating increased intracellular IFN-γ and IL-4 levels. Together, these results present new insights for NDV VLPs induced DC maturation and migration, providing a better understanding of VLP-triggered innate immune responses.