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A Genetic Method To Evaluate the Prevalence of Unique DNA Profiles between Sequential Ground Beef Batches

Author:
Peters, G., Cleveland, B., Higgins, J., Butler, F., Meghen, C.
Source:
Journal of food protection 2017 v.80 no.3 pp. 425-430
ISSN:
0362-028X
Subject:
DNA fingerprinting, Shiga toxin-producing Escherichia coli, cross contamination, genotyping, grinding, ground beef, issues and policy, meat production, models, raw materials, single nucleotide polymorphism
Abstract:
The delineation of ground beef batches has implications for the management of product disposition policies in the event of Shiga toxin–producing Escherichia coli contamination. Analysis of individual contributor animal-specific DNA profiles can provide valuable empirical data for understanding the dynamics of ground meat production processes and can act as a surrogate for cross-contamination. A genetic method was developed for characterizing the source raw material flow and carryover between discrete batches of ground beef in a large-scale commercial beef grinding operation. The application developed involves the introduction of a genetically distinct source raw material batch into the grinding system and comprehensive sampling of that index batch and subsequent batches followed by single nucleotide polymorphism genotyping of random subsamples. Capture-mark-recapture statistical techniques were used to estimate (i) the number of carcass contributors and (ii) the associated level of carryover between batches. Carryover, expressed as a percentage of the total weight of the batch material (in pounds), was observed between the genetically distinct index batch and the next sequential batch at approximately 1%. The nondetection of additional carryover to subsequent batches, with a detection level of approximately 0.2%, supports a serial dilution model of same source raw material carryover, consistent with the recorded weight of beef trimmings used in each batch. For ground beef manufacturers, this method is a simple approach for validating the independence of finished batches of beef in their grind systems in support of product disposition policies.
Agid:
5653157