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Characteristics and proteomic analysis of pyrene degradation by Brevibacillus brevis in liquid medium

Wei, Kun, Yin, Hua, Peng, Hui, Liu, Zehua, Lu, Guining, Dang, Zhi
Chemosphere 2017 v.178 pp. 80-87
Brevibacillus brevis, bioremediation, biosynthesis, carcinogenicity, catalase, ecosystems, energy metabolism, mutagenicity, oxidative stress, physiological response, polycyclic aromatic hydrocarbons, protein synthesis, proteins, proteome, proteomics, superoxide dismutase, two-dimensional gel electrophoresis, unsaturated fatty acids
Polycyclic aromatic hydrocarbons (PAHs) are widely spread in various ecosystems and are of great concern due to their potential toxicity, mutagenicity and carcinogenicity. Bioremediation has been proposed as an effective approach to remove PAHs. In this study, the physiological responses and proteome of Brevibacillus brevis under exposure to pyrene, a four-ring compound from PAHs family, were investigated. The changes of cell viability of B. brevis were observed during the degradation of pyrene by means of flow cytometry. The results indicated that pyrene stimulated superoxide dismutase (SOD) activity from 93.9 to 100.6 U mg−1 prot, whereas inhibited catalase (CAT) activity from 29.1 to 20.3 U mg−1 prot. The main compositions of B. brevis changed during pyrene degradation, with the proportion of unsaturated fatty acids increased by 13.4%. In addition, we performed a proteomic approach (two-dimensional gel electrophoresis and MALDI-TOF/TOF-MS) in order to explore how B. brevis survived upon treatment with pyrene. It was showed that the expression of 13 proteins increased whereas 10 other decreased after pyrene-treatment. The differentially expressed proteins were identified and the results indicated that they were involved in multiple biological processes including energy metabolism, biosynthesis, transmembrane transport and oxidative stress. Overall, these findings offered a new insights into the cellular response strategy developed by B. brevis to overcome the pyrene stress.