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Fractionation of UV-B absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography
- Boulho, R., Le Roux, J., Le Quémener, C., Audo, G., Bourgougnon, N., Bedoux, G.
- Phytochemistry letters 2017 v.20 pp. 410-414
- 2,2-diphenyl-1-picrylhydrazyl, Solieria, absorption, antioxidant activity, chemical elements, countercurrent chromatography, ethyl acetate, fractionation, free radical scavengers, high performance liquid chromatography, macroalgae, methanol, solvents, ultraviolet radiation, Arizona
- Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310nm.