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Fractionation of UV-B absorbing molecules and of free radical scavenging compounds from Solieria chordalis by using centrifugal partition chromatography

Boulho, R., Le Roux, J., Le Quémener, C., Audo, G., Bourgougnon, N., Bedoux, G.
Phytochemistry letters 2017 v.20 pp. 410-414
2,2-diphenyl-1-picrylhydrazyl, Solieria, absorption, antioxidant activity, chemical elements, countercurrent chromatography, ethyl acetate, fractionation, free radical scavengers, high performance liquid chromatography, macroalgae, methanol, solvents, ultraviolet radiation, Arizona
Liquid-liquid chromatography can lead to the isolation of compounds or enrichment of fractions. To investigate the structural characteristics of antioxidants and UV-B absorbing molecules, a preparative centrifugal partition chromatography (CPC) method was developed to prepare enriched bioactive fractions from the red seaweed Solieria chordalis (Gigartinales, Solieriaceae). After a methanolic extraction of dried Solieria chordalis and liquid-liquid separation using ethyl acetate/water (v/v), the ethyl acetate phase was submitted to CPC using Arizona Y of the quaternary solvent system composed of n-heptane/ethyl acetate/methanol/water (19/1/19/1, v/v) in ascending mode. Among the fifteen collected fractions, three fractions gave up to 23.50% of DPPH radical scavenging activity. The CPC fractionation was monitored by HPLC and, four compounds exhibited UV-B absorbing capacity regarding their absorption at 310nm.