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Tissue distribution and functional analysis of vitellogenin-6 of Toxocara canis

Zhu, Hong-Hong, Ma, Guang-Xu, Luo, Yong-Fang, Luo, Yong-Li, Yin, Sha-Sha, Xiong, Yi, Zhou, Rong-Qiong
Experimental parasitology 2017 v.177 pp. 22-27
Canidae, Toxocara canis, adults, amino acids, eggs, embryogenesis, females, genes, germ cells, immunohistochemistry, integument, intestines, lipids, males, polyclonal antibodies, quantitative polymerase chain reaction, rabbits, reproduction, tissue distribution, toxocariasis, transcription (genetics), vitellogenin
Toxocara canis is an common intestinal nematode of canids and the principal causative agent of human toxocariasis. Vitellogenin (Vg), a source of amino acids and lipids in the eggs, are considered to play an important role in embryo development of a wide range of organisms. In the present study, the transcriptional levels of Tc-vit-6 gene in male and female adult T. canis were determined by quantitative real-time PCR, which indicated high transcription of Tc-vit-6 in the intestine, reproductive tract and body wall of male and female adult T. canis. The fragment of Tc-vit-6 encoding a vWD domain, was cloned and expressed to produce a rabbit anti-TcvWD polyclonal antibody. Tissue distribution of TcVg6 was detected by immunohistochemical assays, which showed predominant distribution of TcVg6 in the tissues of intestine, as well as reproductive tract (including some of the germ cells) and musculature of male and female adult worms. Collectively, these results indicated multiple biological roles of TcVg6 apart from that in the reproduction of T. canis.