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Characterization of fungi resistance in two autotetraploid apple cultivars

Chen, Mengmeng, Wang, Feng, Zhang, Zhihong, Fu, Junfan, Ma, Yue
Scientia horticulturae 2017 v.220 pp. 27-35
Alternaria alternata, Glomerella cingulata, Malus domestica, apples, autotetraploidy, biotic stress, breeding, cultivars, diploidy, disease incidence, disease resistance, evolution, fungi, gene expression regulation, genes, industry, quantitative polymerase chain reaction, woody plants
Polyploidy is a key innovation in plant evolution that allows plants to adapt to various biotic stresses. Autotetraploid apple trees (Malus×domestica) perform better in the field than do diploid apple trees. Fungal diseases seriously affect the apple industry, and due to the long growth period of autopolyploid woody plants, little information is available on breeding disease-resistant artificially induced autotetraploid. The study aimed to evaluate the difference in disease resistance between autotetraploid and diploid apple plants and the difference in the expression levels of related genes that may be involved in disease resistance.Two autotetraploid apple cultivars, ‘Hanfu’ and ‘Gala’, perform better in response to Alternaria alternata and Colletotrichum gloeosporioides in that disease spot is smaller, infection is lighter and disease incidence is lower than those in the diploid. Real-time quantitative PCR analysis revealed that disease resistance-related genes were remarkably up-regulated in autotetraploid plants. These results could provide a theoretical basis for breeding disease-resistant autopolyploid apple plants.