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Antioxidant/antihyperglycemic activity of phenolics from sugarcane (Saccharum officinarum L.) bagasse and identification by UHPLC-HR-TOFMS
- Zheng, Rui, Su, Shan, Zhou, Huifang, Yan, Huaifeng, Ye, Junhua, Zhao, Zhengang, You, Lijun, Fu, Xiong
- Industrial crops and products 2017 v.101 pp. 104-114
- 2,2-diphenyl-1-picrylhydrazyl, Saccharum officinarum, alpha-glucosidase, antioxidant activity, antioxidants, genistein, genistin, glucose, glycemic effect, human cell lines, income, p-coumaric acid, quercetin, raw materials, sucrose alpha-glucosidase, sugarcane, sugarcane bagasse
- Sugarcane bagasse, one of the most abundant agro-food by-products, is a very promising raw material available at low cost for recovering bioactive substances. In this study, the total phenolic content was measured by Folin-Ciocalteu method and the antioxidant activity of the extracts was determined by DPPH, ABTS+ and ROO (ORAC) methods. The results indicated that 30% hydroalcoholic fraction (30%E) had the highest total phenolic content (170.68±3.25mg GAE/g DW) among the hydroalcoholic extracts and was the most active fraction in antioxidant activity. Besides, intestinal α-glucosidase inhibitory activity was conducted to assess the antihyperglycemic ability of the extracts and 30%E showed a significant effect at 10mg/mL (82.64% inhibition of sucrase and 74.26% inhibition of maltase, respectively). Glucose uptake assay based on HepG2 cells was used to evaluate the glucose uptake effect of 30%E. Five phenolic compounds (tricin 4-O-guaiacylglyceryl ether-7-O-glucopyranoside, genistin, p-coumaric acid, quercetin and genistein) were identified in 30%E using UHPLC-HR-TOFMS. Taken together, the data indicated that sugarcane bagasse can be used as a potential source of bio-active phenolics and may play a role in functional agents exploitation and agro-industrial revenue increase.