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Development of primer set for the identification of fish species in surimi products using denaturing gradient gel electrophoresis

Author:
Noh, Eun Soo, Park, Yeon Jung, Kim, Eun Mi, An, Cheul Min, Park, Jung Youn, Kim, Kyoung-Ho, Song, Jung-Hun, Kang, Jung-Ha
Source:
Food control 2017 v.79 pp. 74-79
ISSN:
0956-7135
Subject:
DNA primers, cytochrome-c oxidase, denaturing gradient gel electrophoresis, fish, genes, genetic markers, minced fish, mitochondria, rapid methods, surimi
Abstract:
The purpose of this study was to develop a DNA marker for the identification of fish species in processed surimi products. A DNA marker was designed based on the mitochondrial cytochrome c oxidase subunit I gene, and fish species in surimi products were identified using a molecular fingerprinting technique, denaturing gradient gel electrophoresis (DGGE); the results were subjected to sequence-based analysis. The DGGE profiles indicated the presence in surimi products of a greater diversity of fish species than reported previously: 20 species belonging to 16 genera were identified. Therefore, our method facilitates the simple and rapid detection and identification of fish species in seafood products produced from minced fish.
Agid:
5658308