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Dithiocarbamate to modify magnetic graphene oxide nanocomposite (Fe3O4-GO): A new strategy for covalent enzyme (lipase) immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD

Author:
Heidarizadeh, Mohammad, Doustkhah, Esmail, Rostamnia, Sadegh, Rezaei, Parisa Fathi, Harzevili, Farshad Darvishi, Zeynizadeh, Behzad
Source:
International journal of biological macromolecules 2017 v.101 pp. 696-702
ISSN:
0141-8130
Subject:
Fourier transform infrared spectroscopy, X-ray diffraction, carboxylic ester hydrolases, energy-dispersive X-ray analysis, enzymatic hydrolysis, glutaraldehyde, graphene oxide, magnetic materials, nanocomposites, scanning electron microscopy, transmission electron microscopy
Abstract:
Immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4/graphene oxide (GO) via a post-modification. This post modification was achieved in alternation to glutaraldehyde post-modification. The activity of immobilized lipase had not a significant loss in the activity while on the other hand, it is simply extractable (by keeping its major activity) from reaction crude by a magnet. Each step of immobilization was carefully monitored by characterization and all were successfully proved. SEM, TEM, XRD, EDX, and FTIR were used to characterize the support and immobilization process.
Agid:
5660315