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Determination and analysis of the genome sequence of Spodoptera littoralis multiple nucleopolyhedrovirus

Jonathan E. Breitenbach, El-Sayed A. El-Sheikh, Robert L. Harrison, Daniel L. Rowley, Michael E. Sparks, Dawn E. Gundersen-Rindal, Holly J. R. Popham
Virus Research 2013 v.171 no.1 pp. 194-208
sequence analysis, translation (genetics), Spodoptera littoralis, Spodoptera frugiperda multiple nucleopolyhedrovirus, open reading frames, Spodoptera litura, Autographa californica multiple nucleopolyhedrovirus, genetic distance, virulence, bioassays, Spodoptera frugiperda, repetitive sequences, genes, genomics, Spodoptera littoralis nucleopolyhedrovirus, Spodoptera litura nucleopolyhedrovirus, mortality, pathogens
The Spodoptera littoralis multiple nucleopolyhedrovirus (SpliMNPV), a pathogen of the Egyptian cotton leaf worm S. littoralis, was subjected to sequencing of its entire DNA genome and bioassay analysis comparing its virulence to that of other baculoviruses. The annotated SpliMNPV genome of 137,998 bp was found to harbor 132 open reading frames and 15 homologous repeat regions. Four unique genes not present in SpltMNPV were identified, as were 14 genes that were absent or translocated by comparison. Bioassay analysis of experimentally infected Spodoptera frugiperda revealed an extended killing time for SpliMNPV as compared to S. frugiperda MNPV (SfMNPV), but a level of mortality similar to that caused by infection with SfMNPV and superior to that of Autographa californica MNPV (AcMNPV). Although extensive similarity was observed between the genome structure and predicted translation products of SpliMNPV and Spodoptera litura MNPV (SpltMNPV), genetic distances between isolates of SpliMNPV and SpltMNPV suggest that they are in fact different species of genus Alphabaculovirus.