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Stability and tick transmission phenotype of gfp- transformed Anaplasma marginale through a complete in vivo infection cycle
- Noh, Susan M., Ueti, Massaro W., Palmer, Guy H., Munderloh, Ulrike G., Felsheim, Roderick F., Brayton, Kelly A.
- Applied and environmental microbiology 2011 v.77 no.1 pp. 330
- Anaplasma marginale, bovine anaplasmosis, cattle, disease incidence, disease reservoirs, disease transmission, disease vectors, genetic transformation, green fluorescent protein, immunocompetence, in vivo studies, pathogens, phenotype, ticks
- We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp-transformed A. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformed A. marginale and the wild type were the same. However, there were significantly lower levels of the transformed A. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission.