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Differences in Vvufgt and VvmybA1 Gene Expression Levels and Phenolic Composition in Table Grape (Vitis vinifera L.) ‘Red Globe’ and Its Somaclonal Variant ‘Pink Globe’

Author:
Bustamante, Luis, Sáez, Vania, Hinrichsen, Patricio, Castro, María H., Vergara, Carola, von Baer, Dietrich, Mardones, Claudia
Source:
Journal of agricultural and food chemistry 2017 v.65 no.13 pp. 2793-2804
ISSN:
1520-5118
Subject:
Vitis vinifera, anthocyanins, antioxidant activity, epicatechin, gene expression, genotype, microsatellite repeats, procyanidins, quercetin, ripening, seeds, single nucleotide polymorphism, table grapes, taste, transcription (genetics), transcription factors
Abstract:
A novel ‘Red Globe’ (RG)-derived grape variety, ‘Pink Globe’ (PG), was described and registered as a new genotype, with earlier ripening and sweeter taste than those of RG. Microsatellite analysis revealed that PG and RG are undifferentiable; however, the PG VvmybA1c contains six single-nucleotide polymorphisms within the coding and noncoding region, possibly related to the reduced VvmybA1 expression levels. Conversely, HPLC-DAD-ESI-MS/MS analysis showed significantly lower anthocyanin content in PG skin than in RG skin, and PG had no detectable trihydroxylated anthocyanins. Total flavonols did not differ between the variants, although some quercetin derivate concentrations were lower in PG. HPLC-FLD analysis revealed slightly higher concentrations of epicatechin and a procyanidin dimer in PG seeds, although the antioxidant capacity of crude extracts from either variety did not differ significantly. These differences, particularly in monomeric anthocyanin content, can be attributed to altered activity of a MYB-type transcription factor, reducing Vvufgt expression.
Agid:
5662166